Not directly. You would need to flash (update) the firmware ROM of the video card with the Mac version of the ROM for the card. I'm not sure if there is a ROM flasher for this card and if the Mac ROM is available on the net. Also, if the card requires a molex power connection you'd have to split the optical drive's power to get power for this card. I assume when you say this is an AGP card, it's an AGP 8x Pro card?