Port 25 is used for SMTP (Simple Mail Transfer Protocol) connections, which is used by almost all mail clients to send eMail, including Mail. This just means the Mail application couldn't connect to the configured SMTP server.
Possible reasons that come to mind:
You SMTP server is down or busy.
You have the wrong SMTP server address configured in Mail's Accounts (possibly your service changed the value, or the account setting was changed?).
You need to use authentication when connecting to your ISP's SMTP server.
You are trying to connect to an SMTP server that doesn't accept connections from outside its own "trusted" network.
You are trying to connect to an SMTP server from an ISP network that doesn't allow you to connect to other SMTP servers.
You have a firewall set up that blocks port 25.